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Home > Occupational Healthcare > DOT > DOT Urine Lab Based

DOT Urine Lab Based Testing

EBI conducts lab based urine drug testing based on the panel specifications required by DOT CFR Part 40 and conducted on a pre-employment, random and post-accident/for basis as required by the DOT.  Samples are collected through one of our 15,000 plus collection sites and clinics.  Each sample is analyzed through a SAMSHA certified laboratory and reviewed by a MRO for accuracy and compliance.  All results are delivered through our state-of-the-art EBI - Drug Testing Management Center for enhanced security.  


The following is a sample of DOT requirements for urine based drug testing:

  • All testing is conducted in conjunction and in compliance with DOT regulations per CFR Part 40.  (§ 40.3) 
  • All DOT tests are 5 panel and includes the analysis of the following drugs:
1.  Marijuana (THC)
2.  Cocaine
3.  Amphetamines
4.  Opiates
5.  Phencyclidine (PCP)

  • Only Federal DOT chain of custody forms may be used to perform DOT collections.  (§40.45) 
  • All urine specimens are analyzed as split specimens 
  • For DOT testing, only urine drug screens are permitted at this time.  There are no exceptions for hair or saliva.  

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